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To measure PIP 3 , FoB were stimulated with 5 g/mL anti-IgM (Jackson Immunoresearch 715-006-020) for the indicated time points, then fixed in 2% FA and permeabilized with 0.1% saponin

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Environmental factors, including temperature and UV, also belong to physical degradation

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Cell Mol Neurobiol 2005;25:25-39.ArticlePubMedPDF 112